Philippe Bastin's Lab
Research unit @Institut Pasteur & Inserm dealing with trypanosomes, cilia and flagella, tsetse flies, intraflagellar transport, imaging by light and electron microscopy,...
research.pasteur.fr/en/team/trypanosome…
- Dear cilia researchers, join us in the heart of the beautiful Vosges mountains to share your latest findings! This year, partnership with @for5547.bsky.social and keynote lectures by @micromotility.bsky.social and @mick-lab.bsky.social Registration is now open www.cfc2025.conferences-pasteur.org
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- Congratulations! 👏👏👏
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- Nice images! Flagella of the top cell are much shorter, is it because they are bent and hidden behind the cell body or does it correspond to another cell cycle stage?
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- Oh no, I hope you'll be able to fix it
- Happy moments ahead of Mark Carrington special symposium tomorrow @uniofcam.bsky.social
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- Congratulations! 👍
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- Congratulations! 👏👏👏
- Superb piece of work!
- I am super excited to share our latest work on the structures of doublet microtubules from trypanosomatid parasites, the causative agents of leishmaniasis, Chagas disease, and African sleeping sickness. tinyurl.com/48sh3xn5
- Brilliant work with unprecedented level of resolution of the trypanosome axoneme!
- Trypanosome doublet microtubule structures reveal flagellum assembly and motility mechanisms | Science www.science.org/doi/10.1126/...
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- Congratulations! 👍
- Exciting opportunity!
- Re-posts appreciated! We have an open PhD position w/ Prof Harold Auradou on #microswimmers/#protists in complex media! Students get to spend at least 12 months in Paris, and will receive their PhD from both Exeter and Paris-Saclay! Apply by 31st Mar. More details: adum.fr/as/ed/voirpr...
- Cilia talking to each other! 🔊
- Last from the archives, scanning #electronmicroscopy pictures of #cilia in #kidney IMCD3 cells (no triton) by Rania and Thierry from @bastinlab.bsky.social. The longest straight cilium ever and two interacting ones!
- Thanks to a new mass spec pipeline for analysis of the #tubulin tail, the team of Julia Chamot-Rooke and Mariette Matondo revealed a few surprises for #trypanosome beta-tubulin: -a new glutamylation site -far less exhaustive detyrosination Preprint here: www.biorxiv.org/content/10.1...
- Stripping mammalian cells was an exciting experience 😉 Transition fibres are even visible on the right image!
- In 2009, Rania Ghossoub in my lab and Thierry Blisnick in @bastinlab.bsky.social decided to apply a protocol used in Trypanosoma to remove membranes and have access to the axoneme, here is what they saw in IMCD3… #cilia
- Great opportunity to join a fantastic lab with one of the most creative PIs!
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- Enjoy!
- Looking forward to know more about the amazing choanoflagellates!
- Excited to announce the 10th "Choanoflagellates & Friends Workshop" over May 20-22 in our own Institut Pasteur in Paris: 2025choanocon.wordpress.com Registrations open til March 15, abstract til March 5. Pre-conference event for students/postdocs on 19/05. Plenty of slots for selected talks/posters!
- A video clip to see #trypanosomes in #3D #FIB-SEM Many thanks to Valérie Zeitoun and Jeanne Fenouil for their patience during the recording and to Adeline Mallet and Manu Majrouh at the UBI of @pasteur.fr www.pasteur.fr/fr/journal-r...
- I forgot to say that the tagged tubulin incorporates very well in the spindle, here after a 4-hour induction (green, magenta is axoneme + transition zone). However, we could not see a defined polarity, in contrast to flagellar or cortical microtubules.
- Solving 25 years of frustration, Daniel Abbühl managed to tag trypanosome tubulin and to express it in an inducible manner, hence revealing flagellum assembly! A tour-de-force! Preprint here: www.biorxiv.org/content/10.1...
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- Thanks Martin, actually we started these experiments with @keithrmatthews.bsky.social and Azadeh Bagherzadeh in Manchester when we were still in Keith's lab! Although we could express different versions of tagged tubulin, they all failed miserably to be incorporated into microtubules.
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- I fully agree! This is such a conceptual and clever paper and has been a source of inspiration for me and many colleagues working on cilia and flagella!
- Solving 25 years of frustration, Daniel Abbühl managed to tag trypanosome tubulin and to express it in an inducible manner, hence revealing flagellum assembly! A tour-de-force! Preprint here: www.biorxiv.org/content/10.1...
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View full threadData confirmed with Halo-tag tagging of radial spoke proteins, experiment performed by our collaborator Vladimir Varga @czechacademy.bsky.social Always reinsuring to reach the same conclusion with orthogonal techniques!
- We propose a model where the new flagellum grows in a linear manner while the old one is locked but shrinks slightly, hence requiring unlocking and compensation after cell division, leading to such multicoloured flagella following induction/deinduction/reinduction of tagged tubulin 😉
- Second, the old flagellum is indeed locked during assembly of the new flagellum, as we proposed previously www.sciencedirect.com/science/arti... rupress.org/jcb/article/...
- Surprise! Some incorporation takes place in old flagella inherited after cell division!
- In contrast to Chlamydomonas, flagellum growth rate is linear
- Great work showing that cilia are worth even more than motility! 👏👏👏
- 🚨New paper out!🚨 Excited to share our study published in Science Translational Medicine. This 5yr project was led by Susan @dutcherlab.bsky.social and Steve Brody. We asked a simple question: why do some PCD patients develop more severe lung disease than others? 1/ www.science.org/doi/10.1126/...
- And was great with 9 talks and close to 100 participants!
- Better with the link 😅 @club-cfc.bsky.social
- Follow our French #cilia network! Only on BlueSky!
- Follow our French #cilia network! Only on BlueSky!
- Great talk of Christine at the #cilia symposium @institutimagine.bsky.social today! proud PI! Many thanks to @alexbnmrh.bsky.social for the organisation!
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- What a fantastic team! Welcome to Bluesky!
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- I remember that great front cover of JCS with the YL1/2 staining!
- Come and celebrate the exceptional career of Mark Carrington (24th March at University of Cambridge)!
- 🚨 Retirement Symposium for Prof Mark Carrington of the University of Cambridge Our #Trypanosoma colleagues may want to attend this symposium in Cambridge on the 𝟐𝟒𝐭𝐡 𝐨𝐟 𝐌𝐚𝐫𝐜𝐡, 2025 (register by the 15th!) - Details at QR code & tinyurl.com/2uw8tryu
- Brilliant work of Maude Leveque with @mila-montpellier.bsky.social Jeremy Vincent among others revealing unexpected features of #tubulin detyrosination in Leishmania, especially in the amastigote stage!
- Pasteur is on Bluesky! 😀
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- Welcome to Bluesky André and Merry Xmas!
- Exciting programme for webinars in parasitology next year, including @pasteur.fr colleagues Gretchen Diffendall (Artur Scherf's lab), Brice Rotureau, Karinna Rubio (Lucy Glover's lab) and Chetan Chitnis, stay tuned!
- We are pleased to announce the ParaFrap Webinar program for 2025. Join us labex-parafrap.fr/en/conferenc... The ParaFrap network wishes you a happy holiday season.
- How do #trypanosomes restrict #IFT to only 4 doublet microtubules? Two hypotheses were proposed (restriction @ the flagellum base or @ the injection) but in this preprint, Aline Alves reveals it is a third way! It all happens in the proximal portion of the flagellum! www.biorxiv.org/content/10.1...
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View full threadMany thanks to David Perez-Morga for providing FIB-SEM volume, @centriolelab.bsky.social and particularly Vincent Louvel for training on UExM, to Gael Moneron for STED and to UBI and PBI for access to their equipment.
- Feedback is welcome! www.biorxiv.org/content/10.1...
- By FIB-SEM, Aline found multiple densities resembling IFT but thinner and shorter than IFT trains encountered on external portions of flagella. Strikingly, they appear to fuse (or split?) and spread along distinct DMT as if they were able to switch from one DMT to another!
- To demonstrate the molecular identity of these densities, Aline used #UExM coupled to STED to reveal that they indeed contain IFT material that displays the same positioning as the densities detected by FIB-SEM
- dSTORM reveals that IFT proteins are found around all 9 doublet microtubules
- Inspiring discussions with paralympic athletes Mathieu Thomas, Esteban Herrault, Nantenin Keita and Tresor Makunda @pasteur.fr, raising fascinating parallelisms with the challenges we are facing as scientists
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- Happy birthday young man! 🥂
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- Welcome to Bluesky!
- Exciting PhD project in the lab of Sue Vaughan at #Oxford Brookes University to study #trypanosome basal bodies and flagellum formation www.jobs.ac.uk/job/DKY713/n...
- Congratulations to Jean Marc @tsagmoj.bsky.social who won an Early Career Entomologist Award from the @royentsoc.bsky.social for his PhD work on tsetse fly midgut composition and its possible impact on #trypanosome transmission! 💪 Read more here: www.royensoc.co.uk/res-journal-...
